Determination of Urinary Hydroxyl PAHs Using Graphene Oxide@Diatomite Based Solid-Phase Extraction and High-Performance Liquid Chromatography.
Molecules
; 24(22)2019 Nov 19.
Article
em En
| MEDLINE
| ID: mdl-31752256
ABSTRACT
A diatomite supported graphene oxide composite (GO@Dt-NH2) was fabricated and explored as a solid-phase extraction adsorbent coupled with high performance liquid chromatography to determine the trace hydroxyl polycyclic aromatic hydrocarbons (2-hydroxy-naphthalene, 2-hydroxy-fluorene, 1-hydroxy-phenanthrene, and 1-hydroxy-pyrene) in urine samples. The fabricated composites were characterized by X-ray powder diffractometry and scanning electron microscopy. GO@Dt-NH2 offered enhanced adsorption affinity towards the analytes compared with the bare diatomite. The amount of graphene oxide and the factors affecting solid-phase extraction were investigated in detail. Under the optimized conditions, the method gave good linearity (0.30-200 ng/mL) and a low detection limit (0.10-0.15 ng/mL) for the hydroxyl polycyclic aromatic hydrocarbons. The average recovery for spiked urine samples with three levels ranged from 90.6% to 100%. The intra-day and inter-day relative standard deviations were in the range of 1.8-6.4% and 2.7-11.8%, respectively. Besides, the GO@Dt-NH2 provided enrichment factors of 18-20 and superior purification ability. The developed method was successfully applied to the determination of hydroxyl polycyclic aromatic hydrocarbons in urine samples from smoking volunteers.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hidrocarbonetos Policíclicos Aromáticos
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Extração em Fase Sólida
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Grafite
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Hidrocarbonetos
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Terra de Diatomáceas
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article