A custom-built low-cost chamber for exposing rodents to e-cigarette aerosol: practical considerations.
Inhal Toxicol
; 31(11-12): 399-408, 2019.
Article
em En
| MEDLINE
| ID: mdl-31797690
ABSTRACT
Objectives:
To (1) design and build a low-cost exposure chamber system for whole-body exposure of rodents to electronic cigarette aerosol, (2) provide detailed instructions with particular focus on automated e-cigarette activation, and (3) develop a simple mathematical model for aerosol levels in the exposure chamber.Methods:
We built the system with standard laboratory equipment and an open-source electronics platform (Arduino) for e-cigarette activation. Arduino is used to control a solenoid, which pushes the activation button of so-called "Mod" e-cigarettes, and a pump to move the aerosol from the mouthpiece of the e-cigarette into the chamber. For "Pods" and "Cigalikes," the solenoid is not used as they are activated by the vacuum created by the pump. Aerosol concentrations were measured with a light-scattering laser photometer.Results:
The system allows varying the air exchange rate, monitoring aerosol levels, and programing arbitrary puff topography. Aerosol concentrations observed for different chamber operating conditions (puff time and period, e-cigarette power output, air exchange rate) were consistent with the mathematical model.Conclusions:
Our low-cost exposure chamber can be used in animal experimental studies of the health effects of e-cigarettes. Our model allows estimating design parameters such as chamber volume, air exchange rate, and puff period.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Administração por Inalação
/
Aerossóis
/
Desenho de Equipamento
/
Sistemas Eletrônicos de Liberação de Nicotina
/
Nicotina
Tipo de estudo:
Health_economic_evaluation
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article