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Multiscale 3D-printing of microfluidic AFM cantilevers.
Kramer, Robert C L N; Verlinden, Eleonoor J; Angeloni, Livia; van den Heuvel, Anita; Fratila-Apachitei, Lidy E; van der Maarel, Silvère M; Ghatkesar, Murali K.
Afiliação
  • Kramer RCLN; Department of Precision and Microsystems Engineering (PME), Faculty of Mechanical, Maritime, and Materials Engineering (3mE), Delft University of Technology, Mekelweg 2, 2628CD Delft, The Netherlands. m.k.ghatkesar@tudelft.nl.
  • Verlinden EJ; Department of Precision and Microsystems Engineering (PME), Faculty of Mechanical, Maritime, and Materials Engineering (3mE), Delft University of Technology, Mekelweg 2, 2628CD Delft, The Netherlands. m.k.ghatkesar@tudelft.nl.
  • Angeloni L; Department of Precision and Microsystems Engineering (PME), Faculty of Mechanical, Maritime, and Materials Engineering (3mE), Delft University of Technology, Mekelweg 2, 2628CD Delft, The Netherlands. m.k.ghatkesar@tudelft.nl and Department of Biomechanical Engineering (BME), Faculty of Mechanical,
  • van den Heuvel A; Department of Human Genetics, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
  • Fratila-Apachitei LE; Department of Biomechanical Engineering (BME), Faculty of Mechanical, Maritime, and Materials Engineering (3mE), Delft University of Technology, The Netherlands.
  • van der Maarel SM; Department of Human Genetics, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
  • Ghatkesar MK; Department of Precision and Microsystems Engineering (PME), Faculty of Mechanical, Maritime, and Materials Engineering (3mE), Delft University of Technology, Mekelweg 2, 2628CD Delft, The Netherlands. m.k.ghatkesar@tudelft.nl.
Lab Chip ; 20(2): 311-319, 2020 01 21.
Article em En | MEDLINE | ID: mdl-31808485
Microfluidic atomic force microscopy (AFM) cantilever probes have all the functionalities of a standard AFM cantilever along with fluid pipetting. They have a channel inside the cantilever and an aperture at the tip. Such probes are useful for precise fluid manipulation at a desired location, for example near or inside cells. They are typically made by complex microfabrication process steps, resulting in expensive probes. Here, we used two different 3D additive manufacturing techniques, stereolithography and two-photon polymerization, to directly print ready-to-use microfluidic AFM cantilever probes. This approach has considerably reduced the fabrication time and increased the design freedom. One of the probes, 564 µm long, 30 µm wide, 30 µm high, with a 25 µm diameter channel and 2.5 µm wall thickness had a spring constant of 3.7 N m-1 and the polymer fabrication material had an elastic modulus of 4.2 GPa. Using these 3D printed probes, AFM imaging of a surface, puncturing of the cell membrane, and aspiration at the single cell level have been demonstrated.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sondas Moleculares / Dispositivos Lab-On-A-Chip / Impressão Tridimensional Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sondas Moleculares / Dispositivos Lab-On-A-Chip / Impressão Tridimensional Idioma: En Ano de publicação: 2020 Tipo de documento: Article