Neural crest cells bulldoze through the microenvironment using Aquaporin 1 to stabilize filopodia.
Development
; 147(1)2020 01 09.
Article
em En
| MEDLINE
| ID: mdl-31826865
ABSTRACT
Neural crest migration requires cells to move through an environment filled with dense extracellular matrix and mesoderm to reach targets throughout the vertebrate embryo. Here, we use high-resolution microscopy, computational modeling, and in vitro and in vivo cell invasion assays to investigate the function of Aquaporin 1 (AQP-1) signaling. We find that migrating lead cranial neural crest cells express AQP-1 mRNA and protein, implicating a biological role for water channel protein function during invasion. Differential AQP-1 levels affect neural crest cell speed and direction, as well as the length and stability of cell filopodia. Furthermore, AQP-1 enhances matrix metalloprotease activity and colocalizes with phosphorylated focal adhesion kinases. Colocalization of AQP-1 with EphB guidance receptors in the same migrating neural crest cells has novel implications for the concept of guided bulldozing by lead cells during migration.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pseudópodes
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Movimento Celular
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Aquaporina 1
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Crista Neural
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article