Your browser doesn't support javascript.
loading
IL-15 and CD155 expression regulate LAT expression in murine DNAM1+ NK cells, enhancing their effectors functions.
Luu, Thuy T; Wagner, Arnika K; Schmied, Laurent; Meinke, Stephan; Freund, Jacquelyn E; Kambayashi, Taku; Ravens, Inga; Achour, Adnane; Bernhardt, Gunter; Chambers, Benedict J; Höglund, Petter; Kadri, Nadir.
Afiliação
  • Luu TT; Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden.
  • Wagner AK; Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden.
  • Schmied L; Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden.
  • Meinke S; Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden.
  • Freund JE; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Kambayashi T; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Ravens I; Institute of Immunology, Building 11, Hannover Medical School, Hannover, Germany.
  • Achour A; Science for Life Laboratory, Department of Medicine Solna, Karolinska Institute, and Division of Infectious Diseases, Karolinska University Hospital, Solna, Stockholm, Sweden.
  • Bernhardt G; Institute of Immunology, Building 11, Hannover Medical School, Hannover, Germany.
  • Chambers BJ; Center for Infectious Medicine, Department of Medicine, Huddinge, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
  • Höglund P; Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden.
  • Kadri N; Clinic for Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, Sweden.
Eur J Immunol ; 50(4): 494-504, 2020 04.
Article em En | MEDLINE | ID: mdl-31834938
NK cells are innate immune cells characterized by their ability to spontaneously lyse tumor and virally infected cells. We have recently demonstrated that IL-15-sufficient DC regulate NK cell effector functions in mice. Here, we established that among ITAM-proximal signaling molecules, the expression levels of the scaffold molecule Linker for Activation of T cells (LAT) and its transcription factor ELF-1 were reduced 4 days after in vivo depletion of DC. Addition of IL-15, a cytokine presented by DC to NK cells, regulates LAT expression in NK cells with a significant effect on the DNAM1+ subset compared to DNAM1- cells. We also found that LAT expression is regulated via interaction of the DNAM1 receptor with its ligand CD155 in both immature and mature NK cells, independently of NK cell education. Finally, we found that LAT expression within DNAM1+ NK cells might be responsible for enhanced calcium mobilization following the triggering of activating receptors on NK cells. Altogether, we found that LAT expression is tightly regulated in DNAM1+ NK cells, via interaction(s) with DC, which express CD155 and IL-15, resulting in rapid activation of the DNAM1+ subset during activating receptor triggering.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Receptores Virais / Células Matadoras Naturais / Interleucina-15 / Transportador 1 de Aminoácidos Neutros Grandes / Proteínas Adaptadoras de Transdução de Sinal Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Receptores Virais / Células Matadoras Naturais / Interleucina-15 / Transportador 1 de Aminoácidos Neutros Grandes / Proteínas Adaptadoras de Transdução de Sinal Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article