B lymphocytopenia and Bregs in a not-to-die murine sepsis model.
Biochem Biophys Res Commun
; 523(1): 202-207, 2020 02 26.
Article
em En
| MEDLINE
| ID: mdl-31843193
ABSTRACT
Sepsis is a leading cause of mortality in intensive care units due to multi-organ failure caused by dysregulated immune reactions. In this study, kinetic changes in the immune system were analyzed for 72 h in cecal ligation and puncture (CLP)-induced septic mice while preventing animal death by keeping body temperature. Increase of myeloid cells and decrease of B cells in circulation at 6 h after CLP were markedly observed. At the same time point, interleukin (IL)-10 expressing CD5+ regulatory B cells (Bregs) appeared. IL-10 and programmed death-ligand 1 (PD-L1) mRNA as well as IL-1ß, IL-6 and interferon γ (IFNγ) mRNA was increased in the spleen at 6 h. A gradual decrease in Bcl-2 and abrupt increase of Bim expression in the spleen at the late phase were also found. These results showed that B lymphocytopenia with the appearance of Bregs is the earliest event, likely leading to immunoparalysis in sepsis.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sepse
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Modelos Animais de Doenças
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Linfócitos B Reguladores
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Linfopenia
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article