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Chemical Synthesis of Native S-Palmitoylated Membrane Proteins through Removable-Backbone-Modification-Assisted Ser/Thr Ligation.
Huang, Dong-Liang; Montigny, Cédric; Zheng, Yong; Beswick, Veronica; Li, Ying; Cao, Xiu-Xiu; Barbot, Thomas; Jaxel, Christine; Liang, Jun; Xue, Min; Tian, Chang-Lin; Jamin, Nadège; Zheng, Ji-Shen.
Afiliação
  • Huang DL; High Magnetic Field Laboratory, Chinese Academy of Sciences and Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
  • Montigny C; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
  • Zheng Y; High Magnetic Field Laboratory, Chinese Academy of Sciences and Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
  • Beswick V; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
  • Li Y; Department of Physics, Evry-Val-d'Essonne University, 91025, Evry, France.
  • Cao XX; High Magnetic Field Laboratory, Chinese Academy of Sciences and Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
  • Barbot T; High Magnetic Field Laboratory, Chinese Academy of Sciences and Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
  • Jaxel C; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
  • Liang J; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
  • Xue M; High Magnetic Field Laboratory, Chinese Academy of Sciences and Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
  • Tian CL; High Magnetic Field Laboratory, Chinese Academy of Sciences and Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
  • Jamin N; High Magnetic Field Laboratory, Chinese Academy of Sciences and Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China.
  • Zheng JS; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
Angew Chem Int Ed Engl ; 59(13): 5178-5184, 2020 03 23.
Article em En | MEDLINE | ID: mdl-31846559
The preparation of native S-palmitoylated (S-palm) membrane proteins is one of the unsolved challenges in chemical protein synthesis. Herein, we report the first chemical synthesis of S-palm membrane proteins by removable-backbone-modification-assisted Ser/Thr ligation (RBMGABA -assisted STL). This method involves two critical steps: 1) synthesis of S-palm peptides by a new γ-aminobutyric acid based RBM (RBMGABA ) strategy, and 2) ligation of the S-palm RBM-modified peptides to give the desired S-palm product by the STL method. The utility of the RBMGABA -assisted STL method was demonstrated by the synthesis of rabbit S-palm sarcolipin (SLN) and S-palm matrix-2 (M2) ion channel. The synthesis of S-palm membrane proteins highlights the importance of developing non-NCL methods for chemical protein synthesis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Palmitatos / Peptídeos / Serina / Treonina / Proteínas de Membrana Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Palmitatos / Peptídeos / Serina / Treonina / Proteínas de Membrana Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article