Chemical Synthesis of Native S-Palmitoylated Membrane Proteins through Removable-Backbone-Modification-Assisted Ser/Thr Ligation.
Angew Chem Int Ed Engl
; 59(13): 5178-5184, 2020 03 23.
Article
em En
| MEDLINE
| ID: mdl-31846559
The preparation of native S-palmitoylated (S-palm) membrane proteins is one of the unsolved challenges in chemical protein synthesis. Herein, we report the first chemical synthesis of S-palm membrane proteins by removable-backbone-modification-assisted Ser/Thr ligation (RBMGABA -assisted STL). This method involves two critical steps: 1)â
synthesis of S-palm peptides by a new γ-aminobutyric acid based RBM (RBMGABA ) strategy, and 2)â
ligation of the S-palm RBM-modified peptides to give the desired S-palm product by the STL method. The utility of the RBMGABA -assisted STL method was demonstrated by the synthesis of rabbit S-palm sarcolipin (SLN) and S-palm matrix-2 (M2) ion channel. The synthesis of S-palm membrane proteins highlights the importance of developing non-NCL methods for chemical protein synthesis.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Palmitatos
/
Peptídeos
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Serina
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Treonina
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article