The Adult Murine Intestine is Dependent on Constitutive Laminin-γ1 Synthesis.
Sci Rep
; 9(1): 19303, 2019 12 17.
Article
em En
| MEDLINE
| ID: mdl-31848396
Laminin-γ1 is required for early embryonic development; however, the need for laminin-γ1 synthesis in adulthood is unknown. A global and inducible mouse model of laminin-γ1 deficiency was generated to address this question. Genetic ablation of the Lamc1 gene in adult mice was rapidly lethal. Despite global Lamc1 gene deletion in tamoxifen-induced mutant mice, there was minimal change in total cardiac, pulmonary, hepatic or renal laminin protein. In contrast, laminin-γ1 was significantly depleted in the small intestines, which showed crypt hyperplasia and dissociation of villous epithelium from adjacent mesenchyme. We conclude that the physiologic requirement for laminin-γ1 synthesis in adult mice is dependent on a tissue-specific basal rate of laminin-γ1 turnover that results in rapid depletion of laminin-γ1 in the intestine.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Laminina
/
Desenvolvimento Embrionário
/
Intestinos
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article