High Azole Resistance in Aspergillus fumigatus Isolates from Strawberry Fields, China, 2018.
Emerg Infect Dis
; 26(1): 81-89, 2020 01.
Article
em En
| MEDLINE
| ID: mdl-31855142
ABSTRACT
In 2018, we conducted a cross-sectional study to investigate azole resistance in environmental Aspergillus fumigatus isolates obtained from different agricultural fields in China. Using 63 soil cores, we cultured for azole-resistant A. fumigatus and characterized isolates by their cyp51A gene type, short tandem repeat genotype, and mating type. Of 206 A. fumigatus isolates, 21 (10.2%) were azole resistant. Nineteen of 21 had mutations in their cyp51A gene (5 TR34/L98H, 8 TR34/L98H/S297T/F495I, 6 TR46/Y121F/T289A). Eighteen were cultured from soil samples acquired from strawberry fields, suggesting this soil type is a potential hotspot for azole resistance selection. Twenty resistant isolates were mating type MAT1-1, suggesting asexual sporulation contributed to their evolution. Prochloraz, difenoconazole, and tebuconazole were the most frequently detected fungicides in soil samples with azole-resistant fungus. Our study results suggest that managing the fungicides used in agriculture will help contain the problem of antifungal drug resistance in clinics.
Palavras-chave
Aspergillus fumigatus; China; MAT1-1; STR type; agriculture; antifungal drug resistance; antimicrobial resistance; asexual reproduction; asexual sporulation; azole fungicides; azole resistance; cyp51A; epidemiology; farms; fungi; resistance hotspot; short tandem repeat typing; strawberries; strawberry fields
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Aspergillus fumigatus
/
Fragaria
/
Produção Agrícola
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Fungicidas Industriais
Tipo de estudo:
Observational_studies
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Prevalence_studies
/
Risk_factors_studies
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article