A simple and efficient workflow for generation of knock-in mutations in Jurkat T cells using CRISPR/Cas9.

Borowicz, Pawel; Chan, Hanna; Medina, Daniel; Gumpelmair, Simon; Kjelstrup, Hanna; Spurkland, Anne

Borowicz, Pawel; Chan, Hanna; Medina, Daniel; Gumpelmair, Simon; Kjelstrup, Hanna; Spurkland, Anne.

Afiliação

Borowicz P; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Chan H; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Medina D; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Gumpelmair S; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Kjelstrup H; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Spurkland A; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.

Scand J Immunol ; 91(4): e12862, 2020 Apr.

Article em En | MEDLINE | ID: mdl-31889332

Assuntos

Sistemas CRISPR-Cas; Edição de Genes/métodos; Técnicas de Introdução de Genes/métodos; Linfócitos T; Fluxo de Trabalho; Humanos; Células Jurkat; Mutação

Palavras-chave

CRISPR/Cas9 genome editing; LCK kinase; TSAd adaptor protein

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linfócitos T / Técnicas de Introdução de Genes / Fluxo de Trabalho / Sistemas CRISPR-Cas / Edição de Genes Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google