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Analysis and Differential Expression of Primo Genes Using RNA-Seq and qRT-PCR Experiments.
Shin, Jun-Young; Ji, Jong-Ok; Choi, Sang-Heon; Choi, Da-Woon; An, Ye-Jin; Seo, Jae-Hyeok; Choi, Jong-Gu; Rho, Min-Suk; Lee, Ji Yoon; Yeo, Sujung; Lee, Sang-Suk.
Afiliação
  • Shin JY; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Ji JO; Goodpl Co., Ltd, Wonju, Republic of Korea.
  • Choi SH; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Choi DW; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • An YJ; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Seo JH; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Choi JG; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Rho MS; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Lee JY; Department of Biomedical Science, CHA Stem Cell Institute, CHA University, Pocheon, Gyeonggi-do, Republic of Korea.
  • Yeo S; Department of Meridian and Acupuncture, College of Korean Medicine, Sangji University, Wonju, Republic of Korea.
  • Lee SS; Department of Oriental Biomedical Engineering, Sangji University, Wonju, Republic of Korea. sslee@sangji.ac.kr.
Adv Exp Med Biol ; 1232: 393-399, 2020.
Article em En | MEDLINE | ID: mdl-31893436
Although the existence of the primo vasculature system has been shown in many species, including mice, rats, rabbits and humans, the biological role of this system, including expression of genes and proteins, has not yet been investigated. Especially the transcriptional action by mRNA, which is required for biological action, needs to be studied in primo vasculature biology. Differentially expressed genes in both isolated primo vessels and lymphatic vessels of rabbits were analyzed by RNA sequencing experiments. Primer efficiency and RNA purity of the primo vessels under lipopolysaccharides were confirmed prior to performing real-time qRT-PCR analysis following RNA extraction. We demonstrated that FLT4 was enriched in primo vessels and that several genes, including HSPH1 and EPHB2, were highly expressed in primo vessels compared with lymphatic vessels. Our data show that almost all genes, except HSPA4, were increased or sustained in isolated primo vessels compared with lymphatic vessels (FLT4 2.58 fold, HSPH1 1.83 fold, EPHB2 1.52 fold; whereas HSPA4 decreased 0.50 fold), suggesting primo vessels as a central regulator in diverse physiology. This implies that FLT4, HSPH1, and EPHB2 in high amounts may be involved in the functional activity of primo vessels. Our experimental data show that several genes are highly enriched in primo vessels in the lymphatic vessels of the rabbit.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Vasos Linfáticos / RNA-Seq Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Vasos Linfáticos / RNA-Seq Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article