Conditional control of RNA-guided nucleic acid cleavage and gene editing.
Nat Commun
; 11(1): 91, 2020 01 03.
Article
em En
| MEDLINE
| ID: mdl-31900392
ABSTRACT
Prokaryotes use repetitive genomic elements termed CRISPR (clustered regularly interspaced short palindromic repeats) to destroy invading genetic molecules. Although CRISPR systems have been widely used in DNA and RNA technology, certain adverse effects do occur. For example, constitutively active CRISPR systems may lead to a certain risk of off-target effects. Here, we introduce post-synthetic masking and chemical activation of guide RNA (gRNA) to controlling CRISPR systems. An RNA structure profiling probe (2-azidomethylnicotinic acid imidazolide) is used. Moreover, we accomplish conditional control of gene editing in live cells. This proof-of-concept study demonstrates promising potential of chemical activation of gRNAs as a versatile tool for chemical biology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA
/
RNA Guia de Cinetoplastídeos
/
Edição de Genes
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article