Immune effect of Vibrio harveyi formalin-killed cells vaccine combined with chitosan oligosaccharide and astragalus polysaccharides in âEpinephelus fuscoguttatus×âEpinephelus lanceolatus.
Fish Shellfish Immunol
; 98: 186-192, 2020 Mar.
Article
em En
| MEDLINE
| ID: mdl-31926291
ABSTRACT
Vibrio harveyi is the pathogen causing vibriosis in marine-cultured animals, leading to massive deaths in farmed grouper around the world. It is urgent to develop an effective vaccine to prevent vibriosis. In the previous study, we developed a V. harveyi formalin-killed cells vaccine (FKC), and sought an effective adjuvant for enhancing the immune efficacy of vaccine. In this study, we aimed to evaluate the immune responses and protective effect of FKC combined with chitosan oligosaccharide (COS) or Astragalus polysaccharides (APS) in the pearl gentian grouperâEpinephelus fuscoguttatus × âE. lanceolatus. The results indicated the vaccine triggered a remarkably higher expression levels of IL-1ß, IL-16, TNF-α, MHC-Iα and IgM in the kidney and spleen of groupers post-vaccination. Antibody titers, lysozyme, catalase, superoxide dismutase and total protein were significantly elevated in the vaccinated fish compared with those in the control. The experimental groupers were challenged intraperitoneally by V. harveyi at 35 d post-vaccination, and the relative percentage of survival (RPS) of group FKC + COS, FKC + APS, COS, APS and FKC were 80%, 72%, 52%, 47% and 55%, respectively. These results demonstrated COS and APS was the potential adjuvants for FKC against V. harveyi in aquaculture.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vibrio
/
Vibrioses
/
Bass
/
Vacinas Bacterianas
/
Astrágalo
/
Quitosana
/
Doenças dos Peixes
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article