Serum amyloid A (SAA) mRNA expression in chicken and quails in response to bacterial stress.
Comp Immunol Microbiol Infect Dis
; 69: 101421, 2020 Apr.
Article
em En
| MEDLINE
| ID: mdl-31931454
ABSTRACT
Monitoring of acute phase proteins such as serum amyloid A at gene expression level may provide quick information about immune status of the host and its susceptibility towards common infections. Present study was carried out to evaluate and compare the mRNA expression of SAA gene in Rhode Island Red chicken (RIR) and Japanese quails using real time PCR analysis in response to inactivated Salmonella gallinarum culture. The results showed that expression of SAA gene was approximately 17-33 folds higher in case of birds administered with bacterial culture when compared to un-inoculated controls and expression was higher and quicker in case of quails than RIR chicken. The SAA genes from chicken and quail were cloned and upon sequence analysis it was observed that deduced amino acid sequence of SAA from chicken and quails were having approximately seven percent variation which might have significance in function of this protein in these species.
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Base de dados:
MEDLINE
Assunto principal:
Codorniz
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Estresse Fisiológico
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Proteína Amiloide A Sérica
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RNA Mensageiro
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Expressão Gênica
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Galinhas
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article