Molecular detection of free-living amoebae from Namhangang (southern Han River) in Korea.
Sci Rep
; 10(1): 335, 2020 01 15.
Article
em En
| MEDLINE
| ID: mdl-31942007
ABSTRACT
The free-living amoebae Naegleria spp. and Acanthamoeba spp. exist in the natural environment and are sometimes causal agents of lethal primary amoebic meningoencephalitis (PAM), amoebic keratitis (AK) and granulomatous amebic encephalitis (GAE) in humans, respectively. To ascertain the existence of free-living amoebae in Korea, water samples were collected from the Korean hydrosphere, Namhangang (southern Han River), an active location for water skiing and recreation. Samples underwent two-step filtration and were cultured on non-nutrient agar medium with inactivated E. coli. The remaining samples were subjected to PCR for primarily the 18S small ribosomal RNA gene and gene sequencing. Similarities in 18S rDNA sequences, in comparison with various reference amoebae in GenBank, showed 86~99% homology with N. gruberi, N. philippinensis, N. clarki, A. polyphaga, A. castellannii, and Hartmannella (Vermamoeba) vermiformis. Therefore, this study will be useful for seasonal detection of free-living amoebae from various Korean hydrospheres in future studies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rios
/
Amoeba
Tipo de estudo:
Diagnostic_studies
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article