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Put on Your Para-spectacles: The Development of Optimized CRISPR-Cas13-Based Approaches to Image RNA Dynamics in Real Time.
Davis, Brandon J; O'Connell, Mitchell R.
Afiliação
  • Davis BJ; Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA; Center for RNA Biology, University of Rochester, Rochester, NY 14642, USA.
  • O'Connell MR; Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA; Center for RNA Biology, University of Rochester, Rochester, NY 14642, USA. Electronic address: mitchell_oconnell@urmc.rochester.edu.
Mol Cell ; 77(2): 207-209, 2020 01 16.
Article em En | MEDLINE | ID: mdl-31951545
Live-cell RNA imaging is a powerful approach to observe the real-time dynamics of RNA metabolism. Two recent papers describe an optimized fluorescence-based CRISPR-Cas13 approach to image colocalized or repeat-containing RNAs in real time, as well as demonstrate simultaneous RNA-DNA labeling by using Cas13 and Cas9 in tandem.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas Idioma: En Ano de publicação: 2020 Tipo de documento: Article