Put on Your Para-spectacles: The Development of Optimized CRISPR-Cas13-Based Approaches to Image RNA Dynamics in Real Time.
Mol Cell
; 77(2): 207-209, 2020 01 16.
Article
em En
| MEDLINE
| ID: mdl-31951545
Live-cell RNA imaging is a powerful approach to observe the real-time dynamics of RNA metabolism. Two recent papers describe an optimized fluorescence-based CRISPR-Cas13 approach to image colocalized or repeat-containing RNAs in real time, as well as demonstrate simultaneous RNA-DNA labeling by using Cas13 and Cas9 in tandem.
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1
Base de dados:
MEDLINE
Assunto principal:
RNA
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Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article