Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells.
Mol Cell Endocrinol
; 505: 110729, 2020 04 05.
Article
em En
| MEDLINE
| ID: mdl-31972330
ABSTRACT
The aim of this study was to investigate whether co-culture of human islets with adipose-derived stem cells (ASCs) can improve islet quality and to evaluate which factors play a role in the protective effect of ASCs against islet dysfunction. Islets and ASCs were cultured in three experimental groups for 24 h, 48 h, and 72 h 1) indirect co-culture of islets with ASC monolayer (Islets/ASCs); 2) islets alone; and 3) ASCs alone. Co-culture with ASCs improved islet viability and function in all culture time-points analyzed. VEGFA, HGF, IL6, IL8, IL10, CCL2, IL1B, and TNF protein levels were increased in supernatants of islet/ASC group compared to islets alone, mainly after 24 h. Moreover, VEGFA, IL6, CCL2, HIF1A, XIAP, CHOP, and NFKBIA genes were differentially expressed in islets from the co-culture condition compared to islets alone. In conclusion, co-culture of islets with ASCs promotes improvements in islet quality.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Tecido Adiposo
/
Ilhotas Pancreáticas
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article