Class C1 decoy oligodeoxynucleotide inhibits profibrotic genes expression in rat hepatic stellate cells.
Mol Med Rep
; 21(2): 667-674, 2020 02.
Article
em En
| MEDLINE
| ID: mdl-31974596
ABSTRACT
The aim of the present study was to investigate whether class C1 decoy oligodeoxynucleotides (ODNs) can inhibit the expression of profibrotic genes associated with rat hepatic stellate cell (HSC) activation and hepatic fibrosis. Luciferase reporter assays were performed to test the promoter activities of transforming growth factor (TGF)ß and its downstream target genes following transfection of decoy ODNs and plasmids into HSCT6 cells, and western blot assays were performed to measure the protein expression of those genes following decoy ODN transfection. Class C1 decoy ODNs were confirmed to inhibit the promoter activity of TGFß and its downstream target genes, such as type 1 collagen (COLI)α1, tissue inhibitor of metalloproteinases (TIMP)1 and αsmooth muscle actin by Gaussia luciferase reporter assay, and to further downregulate the expression of TGFß, SMAD3, COLIα1 and TIMP1 by western blotting in activated HSCT6 cells. In conclusion, class C1 decoy ODNs inhibited profibrotic gene expression in rat HSCS by downregulating TGFß signaling.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oligodesoxirribonucleotídeos
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Regulação da Expressão Gênica
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Células Estreladas do Fígado
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Cirrose Hepática
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article