Long-term development of human iPSC-derived pyramidal neurons quantified after transplantation into the neonatal mouse cortex.
Dev Biol
; 461(1): 86-95, 2020 05 01.
Article
em En
| MEDLINE
| ID: mdl-31982375
ABSTRACT
One of the main obstacles for studying the molecular and cellular mechanisms underlying human neurodevelopment in vivo is the scarcity of experimental models. The discovery that neurons can be generated from human induced pluripotent stem cells (hiPSCs) paves the way for novel approaches that are stem cell-based. Here, we developed a technique to follow the development of transplanted hiPSC-derived neuronal precursors in the cortex of mice over time. Using post-mortem immunohistochemistry we quantified the differentiation and maturation of dendritic patterns of the human neurons over a total of six months. In addition, entirely hiPSC-derived neuronal parenchyma was followed over eight months using two-photon in vivo imaging through a cranial window. We found that transplanted hiPSC-derived neuronal precursors exhibit a "protracted" human developmental programme in different cortical areas. This offers novel possibilities for the sequential in vivo study of human cortical development and its alteration, followed in "real time".
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Piramidais
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Neurogênese
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Células-Tronco Pluripotentes Induzidas
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Córtex Motor
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article