Sphingosine-1-phosphate alleviates irradiation-induced parotid injury in a miniature pig model.
Oral Dis
; 26(5): 920-929, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-32034858
ABSTRACT
OBJECTIVES:
Our aim was to verify the alleviation effect of sphingosine-1-phosphate (S1P) in a miniature pig model. MATERIAL ANDMETHODS:
Thirty male miniature pigs were randomly separated into 10 groups in our experiment. We administered S1P through the parotid duct in a retrograde fashion 2 hr before irradiation (IR). The salivary flow rate and blood flow rate were tested 20 weeks after IR. The apoptotic level was checked at 12, 24 hr and 7 days post-IR.RESULTS:
Twenty weeks after IR, the salivary flow rate of the IR-side parotid gland in IR + S1P group can be maintained at about 40% of the non-IR side, while only 20% was maintained in the IR group. The blood flow rate and microvascular density were significantly higher in the IR + S1P group than in the IR group. The apoptotic level and cleaved caspase-3 expression were downregulated in IR + S1P group, and the ratio of Bcl-2/Bax was increased. The blood flow rate and CD31 level were significantly restored at 12, 24 hr and 7 days post-IR.CONCLUSION:
Sphingosine-1-phosphate may partially alleviate IR-induced parotid dysfunction by decreasing apoptosis of microvascular endothelial cells and maintaining the blood flow rate.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Glândula Parótida
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Lesões por Radiação
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Esfingosina
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Lisofosfolipídeos
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Células Endoteliais
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article