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Identifying Biomarkers in Lymph Node Metastases of Esophageal Adenocarcinoma for Tumor-Targeted Imaging.
de Gouw, D J J M; Rijpkema, M; de Bitter, T J J; Baart, V M; Sier, C F M; Hernot, S; van Dam, G M; Nagtegaal, I D; Klarenbeek, B R; Rosman, C; van der Post, R S.
Afiliação
  • de Gouw DJJM; Department of Surgery, Radboud university medical center, Nijmegen, The Netherlands.
  • Rijpkema M; Department of Radiology and Nuclear Medicine, Radboud university medical center, Nijmegen, The Netherlands.
  • de Bitter TJJ; Department of Pathology, Radboud university medical center, Geert Grooteplein Zuid 30, 6525 GA, Nijmegen, The Netherlands.
  • Baart VM; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands.
  • Sier CFM; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands.
  • Hernot S; Laboratory for In vivo Cellular and Molecular Imaging, Vrije Universiteit Brussel, Brussel, Belgium.
  • van Dam GM; Department of Surgery and department of Nuclear Medicine and Medical Imaging Center, University Medical Center Groningen, Groningen, The Netherlands.
  • Nagtegaal ID; Department of Pathology, Radboud university medical center, Geert Grooteplein Zuid 30, 6525 GA, Nijmegen, The Netherlands.
  • Klarenbeek BR; Department of Surgery, Radboud university medical center, Nijmegen, The Netherlands.
  • Rosman C; Department of Surgery, Radboud university medical center, Nijmegen, The Netherlands.
  • van der Post RS; Department of Pathology, Radboud university medical center, Geert Grooteplein Zuid 30, 6525 GA, Nijmegen, The Netherlands. Chella.vanderpost@radboudumc.nl.
Mol Diagn Ther ; 24(2): 191-200, 2020 04.
Article em En | MEDLINE | ID: mdl-32048177
INTRODUCTION: Tumor-targeted imaging is a promising technique for the detection of lymph node metastases (LNM) and primary tumors. It remains unclear which biomarker is the most suitable target to distinguish malignant from healthy tissue in esophageal adenocarcinoma (EAC). OBJECTIVE: We performed an immunohistochemistry study to identify viable tumor markers for tumor-targeted imaging of EAC. METHODS: We used samples from 72 patients with EAC to determine the immunohistochemical expression of ten potential tumor biomarkers for EAC (carbonic anhydrase IX [CA-IX], carcinoembryonic antigen [CEA], hepatic growth factor receptor, epidermal growth factor receptor, epithelial membrane antigen [EMA], epithelial cell adhesion molecule [EpCAM], human epidermal growth factor receptor 2 [HER-2], urokinase plasminogen activator receptor, vascular endothelial growth factor-A [VEGF-A], and VEGF receptor 2). Immunohistochemistry was performed on tissue microarrays of LNM (n = 48), primary EACs (n = 62), fibrotic tissues (n = 11), nonmalignant lymph nodes (n = 24), and normal esophageal and gastric tissues (n = 40). Tumor marker staining was scored on intensity and percentage of positive cells. RESULTS: EMA and EpCAM showed strong expression in LNM (> 95%) and primary EACs (> 95%). Significant expression was also observed for LNM and EAC using VEGF-A (85 and 92%), CEA (68 and 54%), and CA-IX (4 and 34%). The other tumor biomarkers showed expression of 0-15% for LNM and primary EAC. Except for VEGF-A, nonmalignant lymph node staining was scored as slight or absent. CONCLUSIONS: High expression rates and correlation between LNM in EAC combined with low expression rates in healthy lymph nodes and esophagus tissues were observed for EpCAM and CEA, meaning these are promising targets for tumor-targeted imaging approaches for lymph nodes in patients with EAC.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / Adenocarcinoma / Biomarcadores Tumorais / Análise Serial de Tecidos / Metástase Linfática Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / Adenocarcinoma / Biomarcadores Tumorais / Análise Serial de Tecidos / Metástase Linfática Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2020 Tipo de documento: Article