Molecular Imaging and In Situ Quantitative Profiling of Fatty Acid Synthase with a Chemical Probe.
Anal Chem
; 92(6): 4419-4426, 2020 03 17.
Article
em En
| MEDLINE
| ID: mdl-32053360
ABSTRACT
Cancer cells rely on fatty acid synthase (FASN), a key enzyme for de novo biosynthesis of long chain fatty acids, to sustain their proliferative potential and drive invasion. Unfortunately, conventional FASN assays are technically inadequate for discerning otherwise elusive FASN activity in complex biological milieux, which has hindered progress in the functional study of FASN and development of its inhibitors. Here, we describe a chemical probe with unprecedented selectivity and sensitivity for the labeling of active FASN in living cells, thus demonstrating a new analytical modality for visualizing endogenous FASN activity and exploring opportunities for drug discovery.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácido Graxo Sintases
/
Imagem Óptica
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article