Investigation on the interaction of food colorant Sudan III with bovine serum albumin using spectroscopic and molecular docking methods.
J Environ Sci Health A Tox Hazard Subst Environ Eng
; 55(6): 669-676, 2020.
Article
em En
| MEDLINE
| ID: mdl-32073347
ABSTRACT
Sudan III is a coloring agent used in chemical industries and food additives. This article uses spectroscopic and molecular docking methods to investigate the interaction of Sudan III with bovine serum albumin (BSA) under a physiological condition. Spectroscopic analysis of the emission quenching revealed that the quenching mechanism of BSA by Sudan III was static. The binding sites and constants of Sudan III-BSA complex were observed to be from 0.72 and 6.41 × 102 L·mol-1 to 0.69 and 5.83 × 102 L·mol-1 at 298 and 310 K, respectively. The enthalpy change (ΔH) and entropy change (ΔS) revealed that van der Waals forces and hydrogen bonds stabilized the Sudan III-BSA complex. Energy transfer from tryptophan to Sudan III occurred by a fluorescence resonance energy transfer mechanism, and the r distance (3.32 nm) had been determined. The results of UV-Vis absorption, synchronous, three-dimensional fluorescence, and circular dichroism spectra showed that Sudan III induced conformational changes of BSA. Molecular docking studies revealed that Sudan III situated within subdomain IIA of BSA. A study on the interaction between Sudan III and BSA was of fundamental importance for providing more information about the potential toxicological effect of chemicals at the molecular level.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Compostos Azo
/
Soroalbumina Bovina
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Corantes de Alimentos
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article