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Renal tissue Po2 sensing during acute hemodilution is dependent on the diluent.
Abrahamson, Jessica R; Read, Austin; Chin, Kyle; Mistry, Nikhil; Joo, Hannah; Desjardins, Jean-Francois; Liu, Elaine; Thai, Kerri; Wilson, David F; Vinogradov, Sergei A; Maynes, Jason T; Gilbert, Richard E; Connelly, Kim A; Baker, Andrew J; Mazer, C David; Hare, Gregory M T.
Afiliação
  • Abrahamson JR; Department of Anesthesia, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
  • Read A; Department of Anesthesia, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
  • Chin K; Department of Anesthesia, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
  • Mistry N; Department of Anesthesia, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
  • Joo H; Institute of Medical Science, University of Toronto, Ontario, Canada.
  • Desjardins JF; Department of Anesthesia, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
  • Liu E; Keenan Research Centre for Biomedical Science in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, Toronto, Ontario, Canada.
  • Thai K; Department of Anesthesia, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
  • Wilson DF; Keenan Research Centre for Biomedical Science in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, Toronto, Ontario, Canada.
  • Vinogradov SA; Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
  • Maynes JT; Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
  • Gilbert RE; Department of Anesthesia and Pain Medicine, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.
  • Connelly KA; Keenan Research Centre for Biomedical Science in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, Toronto, Ontario, Canada.
  • Baker AJ; Department of Medicine, Division of Endocrinology, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
  • Mazer CD; Department of Physiology, University of Toronto, Toronto, Ontario, Canada.
  • Hare GMT; Keenan Research Centre for Biomedical Science in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, Toronto, Ontario, Canada.
Am J Physiol Regul Integr Comp Physiol ; 318(4): R799-R812, 2020 04 01.
Article em En | MEDLINE | ID: mdl-32130021
Sensing changes in blood oxygen content ([Formula: see text]) is an important physiological role of the kidney; however, the mechanism(s) by which the kidneys sense and respond to changes in [Formula: see text] are incompletely understood. Accurate measurements of kidney tissue oxygen tension ([Formula: see text]) may increase our understanding of renal oxygen-sensing mechanisms and could inform decisions regarding the optimal fluid for intravascular volume resuscitation to maintain renal perfusion. In some clinical settings, starch solution may be nephrotoxic, possibly due to inadequacy of tissue oxygen delivery. We hypothesized that hemodilution with starch colloid solutions would reduce [Formula: see text] to a more severe degree than other diluents. Anesthetized Sprague-Dawley rats (n = 77) were randomized to undergo hemodilution with either colloid (6% hydroxyethyl starch or 5% albumin), crystalloid (0.9% saline), or a sham procedure (control) (n = 13-18 rats/group). Data were analyzed by ANOVA with significance assigned at P < 0.05. After hemodilution, mean arterial pressure (MAP) decreased marginally in all groups, while hemoglobin (Hb) and [Formula: see text] decreased in proportion to the degree of hemodilution. Cardiac output was maintained in all groups after hemodilution. [Formula: see text] decreased in proportion to the reduction in Hb in all treatment groups. At comparably reduced Hb, and maintained arterial oxygen values, hemodilution with starch resulted in larger decreases in [Formula: see text] relative to animals hemodiluted with albumin or saline (P < 0.008). Renal medullary erythropoietin (EPO) mRNA levels increased more prominently, relative to other hypoxia-regulated molecules (GLUT-1, GAPDH, and VEGF). Our data demonstrate that the kidney acts as a biosensor of reduced [Formula: see text] following hemodilution and that [Formula: see text] may provide a quantitative signal for renal cellular responsiveness to acute anemia. Evidence of a more severe reduction in [Formula: see text] following hemodilution with starch colloid solution suggests that tissue hypoxia may contribute to starch induced renal toxicity.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oxigênio / Derivados de Hidroxietil Amido / Rim Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oxigênio / Derivados de Hidroxietil Amido / Rim Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article