Genomic Repeats Categorize Genes with Distinct Functions for Orchestrated Regulation.
Cell Rep
; 30(10): 3296-3311.e5, 2020 03 10.
Article
em En
| MEDLINE
| ID: mdl-32160538
ABSTRACT
Repetitive elements are abundantly distributed in mammalian genomes. Here, we reveal a striking association between repeat subtypes and gene function. SINE, L1, and low-complexity repeats demarcate distinct functional categories of genes and may dictate the time and level of gene expression by providing binding sites for different regulatory proteins. Importantly, imaging and sequencing analysis show that L1 repeats sequester a large set of genes with specialized functions in nucleolus- and lamina-associated inactive domains that are depleted of SINE repeats. In addition, L1 transcripts bind extensively to its DNA in embryonic stem cells (ESCs). Depletion of L1 RNA in ESCs leads to relocation of L1-enriched chromosomal segments from inactive domains to the nuclear interior and de-repression of L1-associated genes. These results demonstrate a role of L1 DNA and RNA in gene silencing and suggest a general theme of genomic repeats in orchestrating the function, regulation, and expression of their host genes.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Sequências Repetitivas de Ácido Nucleico
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Genoma
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Regulação da Expressão Gênica no Desenvolvimento
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article