Probing eukaryotic genome functions with synthetic chromosomes.
Exp Cell Res
; 390(1): 111936, 2020 05 01.
Article
em En
| MEDLINE
| ID: mdl-32165165
ABSTRACT
The ability to redesign and reconstruct a cell at whole-genome level provides new platforms for biological study. The international synthetic yeast genome project-Sc2.0, designed by interrogating knowledge amassed by the yeast community to date, exemplifies how a classical synthetic biology "design-build-test-learn" engineering cycle can effectively test hypotheses about various genome fundamentals. The genome reshuffling SCRaMbLE system implemented in synthetic yeast strains also provides unprecedented diversified resources for genotype-phenotype study and yeast metabolic engineering. Further development of genome synthesis technology will shed new lights on complex biological processes in higher eukaryotes.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Genoma Fúngico
/
Cromossomos Artificiais de Levedura
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article