Continuous Online Protein Quality Monitoring during Perfusion Culture Production Using an Integrated Micro/Nanofluidic System.
Anal Chem
; 92(7): 5267-5275, 2020 04 07.
Article
em En
| MEDLINE
| ID: mdl-32167286
ABSTRACT
We demonstrate a new micro/nanofluidic system for continuous and automatic monitoring of protein product size and quantity directly from the culture supernatant during a high-cell-concentration CHO cell perfusion culture. A microfluidic device enables clog-free cell retention for a bench-scale (350 mL) perfusion bioreactor that continuously produces the culture supernatant containing monoclonal antibodies (IgG1). A nanofluidic device directly monitors the protein size and quantity in the culture supernatant. The continuous-flow and fully automated operation of this nanofluidic protein analytics reduces design complexity and offers more detailed information on protein products than offline and batch-mode conventional analytics. Moreover, chemical and mechanical robustness of the nanofluidic device enables continuous monitoring for several days to a week. This continuous and online protein quality monitoring could be deployed at different steps and scales of biomanufacturing to improve product quality and manufacturing efficiency.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Perfusão
/
Proteínas
/
Nanotecnologia
/
Dispositivos Lab-On-A-Chip
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article