In vitro growth of immature bovine follicles and oocytes.
Reprod Fertil Dev
; 32(2): 1-6, 2019 Jan.
Article
em En
| MEDLINE
| ID: mdl-32188553
ABSTRACT
The limitation in the supply of mature, fertilisable oocytes constitutes a major impediment to increasing the success of assisted reproduction, stem cell derivation and cloning in domestic species. Techniques are being developed to grow immature oocytes invitro that have the potential to increase the supply of oocytes. Mouse oocytes can be cultured from initial stages of development to maturity, and live young have been produced, but for domestic species, such as cows, with long growth periods, invitro systems that allow complete growth of oocytes contained within primordial follicles to maturity is technically challenging and has not yet been achieved. For cows, several culture systems have been developed that support specific developmental stages, but a multistep culture system will be required for complete growth invitro. This review highlights the steps that will be required to achieve the goal of growing oocytes invitro.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Bovinos
/
Técnicas de Cultura de Células
/
Técnicas de Maturação in Vitro de Oócitos
/
Folículo Ovariano
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article