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Bacterial nanocellulose as novel carrier for intestinal epithelial cells in drug delivery studies.
Fey, Christina; Betz, Jana; Rosenbaum, Corinna; Kralisch, Dana; Vielreicher, Martin; Friedrich, Oliver; Metzger, Marco; Zdzieblo, Daniela.
Afiliação
  • Fey C; University Hospital Würzburg, Chair Tissue Engineering and Regenerative Medicine (TERM), Würzburg, Germany; Fraunhofer Institute for Silicate Research (ISC), Translational Center Regenerative Therapies (TLC-RT), Würzburg, Germany.
  • Betz J; University Hospital Würzburg, Chair Tissue Engineering and Regenerative Medicine (TERM), Würzburg, Germany.
  • Rosenbaum C; University Hospital Würzburg, Chair Tissue Engineering and Regenerative Medicine (TERM), Würzburg, Germany.
  • Kralisch D; JeNaCell GmbH, Jena, Germany.
  • Vielreicher M; Friedrich-Alexander-University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Department of Chemical and Biological Engineering, Erlangen, Germany.
  • Friedrich O; Friedrich-Alexander-University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Department of Chemical and Biological Engineering, Erlangen, Germany.
  • Metzger M; University Hospital Würzburg, Chair Tissue Engineering and Regenerative Medicine (TERM), Würzburg, Germany; Fraunhofer Institute for Silicate Research (ISC), Translational Center Regenerative Therapies (TLC-RT), Würzburg, Germany.
  • Zdzieblo D; University Hospital Würzburg, Chair Tissue Engineering and Regenerative Medicine (TERM), Würzburg, Germany; Fraunhofer Institute for Silicate Research (ISC), Translational Center Regenerative Therapies (TLC-RT), Würzburg, Germany. Electronic address: daniela.zdzieblo@uni-wuerzburg.de.
Mater Sci Eng C Mater Biol Appl ; 109: 110613, 2020 Apr.
Article em En | MEDLINE | ID: mdl-32228900
ABSTRACT
Synthetic cell carriers (A) represent common scaffold structures for the development of cell-based in vitro models of the human intestine but due to their low porosity or unwanted molecular adhesion effects, synthetic carriers can negatively affect cell function. Alternative scaffolds such as natural extracellular matrices (ECMs) (B) were shown to overcome some of the common drawbacks. However, their fabrication is time-consuming, less well standardized and not entirely conform to the 3R principle (replacement, reduction, refinement). Nowadays, biopolymers such as bacterial nanocellulose (BNC) (C) represent interesting scaffold materials for innovative tissue engineering concepts, as they can be generated in a faster and more standardized process workflow without the need for animal material. In this study, we demonstrate the BNC as suitable carrier for the development of Caco-2-based in vitro models of the human intestine. The BNC-based models exhibit organ-specific properties comprising typical cellular morphologies, characteristic protein expression profiles, representative ultrastructural features and the formation of a tight epithelial barrier. The proof of in vivo-like transport activities further validates the high quality of the BNC-based Caco-2 models. In summary, this illustrates the BNC as alternative bioscaffold of non-animal origin to develop functional organ models in vitro.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Portadores de Fármacos / Celulose / Nanoestruturas / Células Epiteliais / Mucosa Intestinal Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Portadores de Fármacos / Celulose / Nanoestruturas / Células Epiteliais / Mucosa Intestinal Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article