Differential Scaling of Gene Expression with Cell Size May Explain Size Control in Budding Yeast.
Mol Cell
; 78(2): 359-370.e6, 2020 04 16.
Article
em En
| MEDLINE
| ID: mdl-32246903
ABSTRACT
Yeast cells must grow to a critical size before committing to division. It is unknown how size is measured. We find that as cells grow, mRNAs for some cell-cycle activators scale faster than size, increasing in concentration, while mRNAs for some inhibitors scale slower than size, decreasing in concentration. Size-scaled gene expression could cause an increasing ratio of activators to inhibitors with size, triggering cell-cycle entry. Consistent with this, expression of the CLN2 activator from the promoter of the WHI5 inhibitor, or vice versa, interfered with cell size homeostasis, yielding a broader distribution of cell sizes. We suggest that size homeostasis comes from differential scaling of gene expression with size. Differential regulation of gene expression as a function of cell size could affect many cellular processes.
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MEDLINE
Assunto principal:
Divisão Celular
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Ciclinas
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Proteínas de Saccharomyces cerevisiae
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Tamanho Celular
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article