Visualization of individual microRNA molecules in fixed cells and tissues using target-primed padlock probe assay.
Biochem Biophys Res Commun
; 526(3): 607-611, 2020 06 04.
Article
em En
| MEDLINE
| ID: mdl-32247612
ABSTRACT
MicroRNAs (miRNAs) are key regulators of gene expression at the posttranscriptional level. Precisely profiling of miRNA expression will help us to better understand their roles in normal and diseased cells and tissues. Here we describe in situ miRNA detection by padlock probing and miRNA target-primed rolling circle amplification. We optimized our protocol and showed it can be applied to both fixed cells and tissue sections. The method can be used in basic research and potentially in clinical diagnostics in the future.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Imagem Óptica
Tipo de estudo:
Guideline
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article