Infant Alveolar Macrophages Are Unable to Effectively Contain Mycobacterium tuberculosis.
Front Immunol
; 11: 486, 2020.
Article
em En
| MEDLINE
| ID: mdl-32265931
ABSTRACT
Infants are more likely to develop lethal disseminated forms of tuberculosis compared with older children and adults. The reasons for this are currently unknown. In this study we test the hypothesis that antimycobacterial function is impaired in infant alveolar macrophages (AMÏs) compared with those of adults. We develop a method of obtaining AMÏs from healthy infants using rigid bronchoscopy and incubate the AMÏs with live virulent Mycobacterium tuberculosis (Mtb). Infant AMÏs are less able to restrict Mtb replication compared with adult AMÏs, despite having similar phagocytic capacity and immunophenotype. RNA-Seq showed that infant AMÏs exhibit lower expression of genes involved in mycobactericidal activity and IFNγ-induction pathways. Infant AMÏs also exhibit lower expression of genes encoding mononuclear cell chemokines such as CXCL9. Our data indicates that failure of AMÏs to contain Mtb and recruit additional mononuclear cells to the site of infection helps to explain the more fulminant course of tuberculosis in early life.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Macrófagos Alveolares
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Sistema Imunitário
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Lactente
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Mycobacterium tuberculosis
Limite:
Adult
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Aged
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Humans
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Middle aged
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article