LARP1 isoform expression in human cancer cell lines.
RNA Biol
; 18(2): 237-247, 2021 02.
Article
em En
| MEDLINE
| ID: mdl-32286153
ABSTRACT
LARP1 is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. From published in vitro studies, there is disparity over which of two different LARP1 protein isoforms (termed the long LI-LARP1 and short SI-LARP1) is the canonical. Here, after conducting a series of biochemical and cellular assays, we conclude that LI-LARP1 (NM_033551.3 > NP_056130.2) is the dominantly expressed form. We observe that SI-LARP1 (NM_015315.5> NP_056130.2) is epigenetically repressed and that this repression is evolutionarily conserved in all but a small subclade of mammalian species. As with other LARP family members, there are multiple potential LARP1 mRNA isoforms that appear to be censored within the nucleus. The capacity of the cell to modulate splicing and expression of these apparently 'redundant' mRNAs hints at contextually specific mechanisms of LARP1 expression.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ribonucleoproteínas
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Autoantígenos
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Regulação Neoplásica da Expressão Gênica
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article