Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues.
Opt Lett
; 45(8): 2247-2250, 2020 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-32287205
ABSTRACT
We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
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Imagem Molecular
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Imagem Óptica
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article