Dual-Activatable Cell Tracker for Controlled and Prolonged Single-Cell Labeling.
ACS Chem Biol
; 15(6): 1613-1620, 2020 06 19.
Article
em En
| MEDLINE
| ID: mdl-32298071
ABSTRACT
Cell trackers are fluorescent chemical tools that facilitate imaging and tracking cells within live organisms. Despite their versatility, these dyes lack specificity, tend to leak outside of the cell, and stain neighboring cells. Here, we report a dual-activatable cell tracker for increased spatial and temporal staining control, especially for single-cell tracking. This probe overcomes the typical problems of current cell trackers off-target staining, high background signal, and leakage from the intracellular medium. Staining with this dye is not cytotoxic, and it can be used in sensitive primary cells. Moreover, this dye is resistant to harsh fixation and permeabilization conditions and allows for multiwavelength studies with confocal microscopy and fluorescence-activated cell sorting. Using this cell tracker, we performed in vivo homing experiments in mice with primary splenocytes and tracked a single cell in a heterogeneous, multicellular culture environment for over 20 h. These experiments, in addition to comparative proliferation studies with other cell trackers, demonstrated that the signal from this dye is retained in cells for over 72 h after photoactivation. We envision that this type of probes will facilitate the analysis of single-cell behavior and migration in cell culture and in vivo experiments.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rastreamento de Células
/
Análise de Célula Única
/
Corantes Fluorescentes
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article