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Depauperate major histocompatibility complex variation in the endangered reticulated flatwoods salamander (Ambystoma bishopi).
Williams, Steven Tyler; Haas, Carola A; Roberts, James H; Taylor, Sabrina S.
Afiliação
  • Williams ST; School of Renewable Natural Resources, Louisiana State University AgCenter, Baton Rouge, LA, 70806, USA. TylerWil@VT.edu.
  • Haas CA; Department of Fish and Wildlife Conservation, Virginia Tech, Blacksburg, VA, 24061, USA.
  • Roberts JH; Department of Biology, Georgia Southern University, Statesboro, GA, 30458, USA.
  • Taylor SS; School of Renewable Natural Resources, Louisiana State University AgCenter, Baton Rouge, LA, 70806, USA.
Immunogenetics ; 72(4): 263-274, 2020 05.
Article em En | MEDLINE | ID: mdl-32300829
Reticulated flatwoods salamander (Ambystoma bishopi) populations began decreasing dramatically in the 1900s. Contemporary populations are small, isolated, and may be susceptible to inbreeding and reduced adaptive potential because of low genetic variation. Genetic variation at immune genes is especially important as it influences disease susceptibility and adaptation to emerging infectious pathogens, a central conservation concern for declining amphibians. We collected samples from across the extant range of this salamander to examine genetic variation at major histocompatibility complex (MHC) class Iα and IIß exons as well as the mitochondrial control region. We screened tail or toe tissue for ranavirus, a pathogen associated with amphibian declines worldwide. Overall, we found low MHC variation when compared to other amphibian species and did not detect ranavirus at any site. MHC class Iα sequencing revealed only three alleles with a nucleotide diversity of 0.001, while MHC class IIß had five alleles with a with nucleotide diversity of 0.004. However, unique variation still exists across this species' range with private alleles at three sites. Unlike MHC diversity, mitochondrial variation was comparable to levels estimated for other amphibians with nine haplotypes observed, including one haplotype shared across all sites. We hypothesize that a combination of a historic disease outbreak and a population bottleneck may have contributed to low MHC diversity while maintaining higher levels of mitochondrial DNA variation. Ultimately, MHC data indicated that the reticulated flatwoods salamander may be at an elevated risk from infectious diseases due to low levels of immunogenetic variation necessary to combat novel pathogens.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ambystoma / Complexo Principal de Histocompatibilidade Limite: Animals País como assunto: America do norte Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ambystoma / Complexo Principal de Histocompatibilidade Limite: Animals País como assunto: America do norte Idioma: En Ano de publicação: 2020 Tipo de documento: Article