ß-Hydroxybutyrate dehydrogenase decorated MXene nanosheets for the amperometric determination of ß-hydroxybutyrate.
Mikrochim Acta
; 187(5): 277, 2020 04 20.
Article
em En
| MEDLINE
| ID: mdl-32314063
ABSTRACT
MXene nanosheets of type Ti3C2Tx were modified with ß-hydroxybutyrate dehydrogenase and then used as a biosensor for amperometric sensing of ß-hydroxybutyrate. The MXene and the nanocomposite were characterized by X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The MXene has a layered structure and proved to be an excellent immobilization matrix providing good compatibility with the enzyme ß-hydroxybutyrate dehydrogenase. The MXene-based biosensor, best operated at a potential of - 0.35 V (vs. Ag/AgCl), displays a wide linear range (0.36 to 17.9 mM), a sensitivity of 0.480 µA mM-1 cm-2, and a low detection limit (45 µM). The biosensor was successfully applied to the determination of ß-hydroxybutyrate in (spiked) real serum samples. Graphical abstract Schematic representation of the synthesis and decoration of Mxene 2D sheets with ß-hydroxybutyrate dehydrogenase for the amperometric determination of ß-hydroxybutyric acid.
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MEDLINE
Assunto principal:
Titânio
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Compostos Inorgânicos de Carbono
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Ácido 3-Hidroxibutírico
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Nanocompostos
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Hidroxibutirato Desidrogenase
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article