Dynamics of heavy chain junctional length biases in antibody repertoires.
Commun Biol
; 3(1): 207, 2020 05 01.
Article
em En
| MEDLINE
| ID: mdl-32358517
ABSTRACT
Antibody variable domain sequence diversity is generated by recombination of germline segments. The third complementarity-determining region of the heavy chain (CDR H3) is the region of highest sequence diversity and is formed by the joining of heavy chain VH, DH and JH germline segments combined with random nucleotide trimming and additions between these segments. We show that CDR H3 and junctional segment length distributions are biased in human antibody repertoires as a function of VH, VL and JH germline segment utilization. Most length biases are apparent in the naive and antigen experienced B cell compartments but not in nonproductive recombination products, indicating B cell selection as a major driver of these biases. Our findings reveal biases in the antibody CDR H3 diversity landscape shaped by VH, VL, and JH germline segment use during naive and antigen-experienced repertoire selection.
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1
Base de dados:
MEDLINE
Assunto principal:
Cadeias Pesadas de Imunoglobulinas
/
Diversidade de Anticorpos
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article