Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.

Lambert, Abigail R; Hallinan, Jazmine P; Werther, Rachel; Glów, Dawid; Stoddard, Barry L

Lambert, Abigail R; Hallinan, Jazmine P; Werther, Rachel; Glów, Dawid; Stoddard, Barry L.

Afiliação

Lambert AR; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N., Seattle, WA 98109, USA.
Hallinan JP; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N., Seattle, WA 98109, USA.
Werther R; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N., Seattle, WA 98109, USA.
Glów D; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N., Seattle, WA 98109, USA; Laboratory of Bioinformatics and Protein Engineering, International Institute of Molecular and Cell Biology, Warsaw, Poland.
Stoddard BL; Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N., Seattle, WA 98109, USA. Electronic address: bstoddar@fredhutch.org.

Structure ; 28(7): 760-775.e8, 2020 07 07.

Article em En | MEDLINE | ID: mdl-32359399

Assuntos

Proteínas de Ligação a DNA/química; DNA/química; Animais; Sítios de Ligação; DNA/genética; DNA/metabolismo; Proteínas de Ligação a DNA/metabolismo; Edição de Genes/métodos; Humanos; Mutação; Ligação Proteica; Engenharia de Proteínas/métodos; Estabilidade Proteica

Palavras-chave

DNA binding proteins; flow cytometry; gene editing; meganucleases; protein engineering; protein stability; selections; surface display

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Proteínas de Ligação a DNA Tipo de estudo: Clinical_trials Limite: Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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