The pathways by which the marine diatom Thalassiosira sp. OUC2 biodegrades p-xylene, combined with a mechanistic analysis at the proteomic level.
Ecotoxicol Environ Saf
; 198: 110687, 2020 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-32361489
ABSTRACT
A marine diatom, Thalassiosira sp. OUC2, was isolated from natural seawater collected from Daya Bay, China. This diatom degraded 1.25-40 mg L-1p-xylene within five days, at a removal efficiency exceeding 98%. Gas chromatography-mass spectrometer (GC-MS) analysis indicated that p-xylene was converted into 4-methylbenzyl alcohol, p-toluic acid, and p-cresol in the presence of strain OUC2. Meanwhile, proteomic analysis showed that, after exposure to p-xylene, several algal enzymes were significantly upregulated including monooxygenase, alcohol dehydrogenase, benzaldehyde dehydrogenase, benzoate 1,2-dioxygenase, and catechol 2,3-dioxygenase. Moreover, ecotoxicological tests suggested that the intermediate metabolites were less toxic than the parent compound (p-xylene). Thalassiosira sp. OUC2 may thus be suitable for the remediation of p-xylene-contaminated marine environments.
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Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
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Xilenos
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Biodegradação Ambiental
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Diatomáceas
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article