Mechanistic Insight into Crossing over during Mouse Meiosis.
Mol Cell
; 78(6): 1252-1263.e3, 2020 06 18.
Article
em En
| MEDLINE
| ID: mdl-32362315
ABSTRACT
Crossover recombination is critical for meiotic chromosome segregation, but how mammalian crossing over is accomplished is poorly understood. Here, we illuminate how strands exchange during meiotic recombination in male mice by analyzing patterns of heteroduplex DNA in recombinant molecules preserved by the mismatch correction deficiency of Msh2-/- mutants. Surprisingly, MSH2-dependent recombination suppression was not evident. However, a substantial fraction of crossover products retained heteroduplex DNA, and some provided evidence of MSH2-independent correction. Biased crossover resolution was observed, consistent with asymmetry between DNA ends in earlier intermediates. Many crossover products yielded no heteroduplex DNA, suggesting dismantling by D-loop migration. Unlike the complexity of crossovers in yeast, these simple modifications of the original double-strand break repair model-asymmetry in recombination intermediates and D-loop migration-may be sufficient to explain most meiotic crossing over in mice while also addressing long-standing questions related to Holliday junction resolution.
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Base de dados:
MEDLINE
Assunto principal:
Troca Genética
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Recombinação Homóloga
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Meiose
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article