High-throughput cell and spheroid mechanics in virtual fluidic channels.
Nat Commun
; 11(1): 2190, 2020 05 04.
Article
em En
| MEDLINE
| ID: mdl-32366850
ABSTRACT
Microfluidics by soft lithography has proven to be of key importance for biophysics and life science research. While being based on replicating structures of a master mold using benchtop devices, design modifications are time consuming and require sophisticated cleanroom equipment. Here, we introduce virtual fluidic channels as a flexible and robust alternative to microfluidic devices made by soft lithography. Virtual channels are liquid-bound fluidic systems that can be created in glass cuvettes and tailored in three dimensions within seconds for rheological studies on a wide size range of biological samples. We demonstrate that the liquid-liquid interface imposes a hydrodynamic stress on confined samples, and the resulting strain can be used to calculate rheological parameters from simple linear models. In proof-of-principle experiments, we perform high-throughput rheology inside a flow cytometer cuvette and show the Young's modulus of isolated cells exceeds the one of the corresponding tissue by one order of magnitude.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
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Microfluídica
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Técnicas Analíticas Microfluídicas
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Dimetilpolisiloxanos
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Módulo de Elasticidade
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article