Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells.
Methods Mol Biol
; 2142: 93-102, 2020.
Article
em En
| MEDLINE
| ID: mdl-32367361
ABSTRACT
In this chapter, we describe production and purification of the Zika virus NS1 glycoprotein in human embryonic kidney (HEK293T) cells at small, research laboratory scale. The expression of secreted NS1 (sNS1) and the C-terminal ß-ladder domain in HEK293T cells were tested in a small-scale transfection before scaling up to a larger-scale transfection using roller bottles. Two different purification approaches have been applied to obtain purified NS1 (sNS1) and the C-terminal ß-ladder domain ready for clinical applications.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Clonagem Molecular
/
Proteínas não Estruturais Virais
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article