Biomimetic aorta-gonad-Mesonephros-on-a-Chip to study human developmental hematopoiesis.
Biomed Microdevices
; 22(2): 34, 2020 05 06.
Article
em En
| MEDLINE
| ID: mdl-32377802
ABSTRACT
A fundamental limitation in the derivation of hematopoietic stem and progenitor cells is the imprecise understanding of human developmental hematopoiesis. Herein we established a multilayer microfluidic Aorta-Gonad-Mesonephros (AGM)-on-a-chip to emulate developmental hematopoiesis from pluripotent stem cells. The device consists of two layers of microchannels separated by a semipermeable membrane, which allows the co-culture of human hemogenic endothelial (HE) cells and stromal cells in a physiological relevant spatial arrangement to replicate the structure of the AGM. HE cells derived from human induced pluripotent stem cells (hiPSCs) were cultured on a layer of mesenchymal stromal cells in the top channel while vascular endothelial cells were co-cultured on the bottom side of the membrane within the microfluidic device. We show that this AGM-on-a-chip efficiently derives endothelial-to-hematopoietic transition (EHT) from hiPSCs compared with regular suspension culture. The presence of mesenchymal stroma and endothelial cells renders functional HPCs in vitro. We propose that the AGM-on-a-chip could serve as a platform to dissect the cellular and molecular mechanisms of human developmental hematopoiesis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Aorta
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Biomimética
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Dispositivos Lab-On-A-Chip
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Gônadas
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Hematopoese
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Mesonefro
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article