Single-Cell Analysis of Human Retina Identifies Evolutionarily Conserved and Species-Specific Mechanisms Controlling Development.
Dev Cell
; 53(4): 473-491.e9, 2020 05 18.
Article
em En
| MEDLINE
| ID: mdl-32386599
ABSTRACT
The development of single-cell RNA sequencing (scRNA-seq) has allowed high-resolution analysis of cell-type diversity and transcriptional networks controlling cell-fate specification. To identify the transcriptional networks governing human retinal development, we performed scRNA-seq analysis on 16 time points from developing retina as well as four early stages of retinal organoid differentiation. We identified evolutionarily conserved patterns of gene expression during retinal progenitor maturation and specification of all seven major retinal cell types. Furthermore, we identified gene-expression differences between developing macula and periphery and between distinct populations of horizontal cells. We also identified species-specific patterns of gene expression during human and mouse retinal development. Finally, we identified an unexpected role for ATOH7 expression in regulation of photoreceptor specification during late retinogenesis. These results provide a roadmap to future studies of human retinal development and may help guide the design of cell-based therapies for treating retinal dystrophies.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retina
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Células Fotorreceptoras Retinianas Cones
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Regulação da Expressão Gênica no Desenvolvimento
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Organogênese
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Fatores de Transcrição Hélice-Alça-Hélice Básicos
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Evolução Biológica
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Análise de Célula Única
Tipo de estudo:
Prognostic_studies
Limite:
Aged80
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Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article