Evolutionarily ancient BAH-PHD protein mediates Polycomb silencing.
Proc Natl Acad Sci U S A
; 117(21): 11614-11623, 2020 05 26.
Article
em En
| MEDLINE
| ID: mdl-32393638
ABSTRACT
Methylation of histone H3 lysine 27 (H3K27) is widely recognized as a transcriptionally repressive chromatin modification but the mechanism of repression remains unclear. We devised and implemented a forward genetic scheme to identify factors required for H3K27 methylation-mediated silencing in the filamentous fungus Neurospora crassa and identified a bromo-adjacent homology (BAH)-plant homeodomain (PHD)-containing protein, EPR-1 (effector of polycomb repression 1; NCU07505). EPR-1 associates with H3K27-methylated chromatin, and loss of EPR-1 de-represses H3K27-methylated genes without loss of H3K27 methylation. EPR-1 is not fungal-specific; orthologs of EPR-1 are present in a diverse array of eukaryotic lineages, suggesting an ancestral EPR-1 was a component of a primitive Polycomb repression pathway.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Homeodomínio
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Evolução Molecular
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Inativação Gênica
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Proteínas do Grupo Polycomb
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article