A novel strategy for rhodamine B-based fluorescent probes with a selective glutathione response for bioimaging in living cells.
Analyst
; 145(12): 4239-4244, 2020 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-32436498
ABSTRACT
The aim of this study was to overcome the reported shortcomings of the glutathione (GSH) detection of rhodamine-based fluorescent probes, such as poor selectivity to thiol groups and reversible unstable covalent binding with the thiol groups. Here, we have developed a simple and specific fluorescent probe based on rhodamine B, which can be used to selectively detect GSH in solution and perform bioimaging in living cells. This design strategy uses a specific reaction between allenamide and the GSH thiol group, which proceeds rapidly in a phosphate buffer/tetrahydrofuran (PBS/THF) mixture with specific selectivity, and forms a stable and irreversible conjugate. The combined simplicity and specificity of the recognition process enables it to serve as a fluorescent probe for detecting GSH level changes in living cells. Such a new recognizing strategy thus may open a new window for its further application in detecting GSH levels both in vitro and in vivo.
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1
Base de dados:
MEDLINE
Assunto principal:
Rodaminas
/
Corantes Fluorescentes
/
Glutationa
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article