Different epigenome regulation and transcriptome expression of CD4+ and CD8+ T cells from monozygotic twins discordant for psoriasis.
Australas J Dermatol
; 61(4): e388-e394, 2020 Nov.
Article
em En
| MEDLINE
| ID: mdl-32441058
ABSTRACT
OBJECTIVES:
Psoriasis is an immunodeficient skin disorder, and its exact pathogenesis is unclear. Monozygotic twins are presumed to be genetically identical, and their phenotypic differences may be due to transcriptional regulation or epigenome factors. To explain the inconsistency between twins, we have collected 3 pairs of monozygotic twins who are discordant for psoriasis.METHODS:
Reduced representation of bisulfite sequencing and RNA sequencing was conducted using the peripheral blood of the twins to find the genes playing important roles in psoriasis pathogenesis.RESULTS:
As a result, we found methylation diversity in four genes (MAST3, MTOR, PM20D1 and ZNF99), and we also found 9 differentially expressed genes (PPAN-P2RY11, PIGV, RPS18, TMEM121, KIF21A, KCNH2, WNT10B, PRX and CDH24) by RNA sequencing. According to the conjoint analysis of methylation and the mRNA results, PTPN6, CCL5, NFATC1 and PRF1 were found to be closely related to psoriasis. We then annotated the genes to explore the associations between these genes and psoriasis.CONCLUSIONS:
These findings provide a better understanding of psoriasis that can improve the diagnosis and treatment of the disease.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Psoríase
/
Gêmeos Monozigóticos
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Linfócitos T CD4-Positivos
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Linfócitos T CD8-Positivos
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article