High cell-density fermentation, expression and purification of bacteriophage lysin TSPphg, a thermostable antimicrobial protein from extremophilic Thermus bacteriophage TSP4.

Wang, Feng; Zhang, Guanling; Peng, Jiani; Ji, Xinyu; Hai, Jun; Deng, Xianyu; Lin, Lianbing

Wang, Feng; Zhang, Guanling; Peng, Jiani; Ji, Xinyu; Hai, Jun; Deng, Xianyu; Lin, Lianbing.

Afiliação

Wang F; Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China.
Zhang G; Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China.
Peng J; Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China.
Ji X; Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China.
Hai J; Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China.
Deng X; Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China.
Lin L; Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China; Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College, 727 South Jingming Road, Chenggong

Protein Expr Purif ; 174: 105676, 2020 10.

Article em En | MEDLINE | ID: mdl-32442498

Assuntos

Anti-Infecciosos; Bacteriófagos; Endopeptidases; Siphoviridae; Thermus/virologia; Proteínas Virais; Anti-Infecciosos/química; Anti-Infecciosos/isolamento & purificação; Anti-Infecciosos/farmacologia; Bacteriófagos/enzimologia; Bacteriófagos/genética; Endopeptidases/biossíntese; Endopeptidases/genética; Endopeptidases/isolamento & purificação; Endopeptidases/farmacologia; Escherichia coli/enzimologia; Escherichia coli/genética; Proteínas Recombinantes/biossíntese; Proteínas Recombinantes/genética; Proteínas Recombinantes/isolamento & purificação; Proteínas Recombinantes/farmacologia; Siphoviridae/enzimologia; Siphoviridae/genética; Proteínas Virais/biossíntese; Proteínas Virais/genética; Proteínas Virais/isolamento & purificação; Proteínas Virais/farmacologia

Palavras-chave

Antimicrobial protein; Bacteriophage; Endolysin; Fed-batch fermentation; High cell-density cultivation

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Endopeptidases / Thermus / Bacteriófagos / Proteínas Virais / Siphoviridae / Anti-Infecciosos Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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