A novel exopolysaccharide-producing and long-chain n-alkane degrading bacterium Bacillus licheniformis strain DM-1 with potential application for in-situ enhanced oil recovery.
Sci Rep
; 10(1): 8519, 2020 05 22.
Article
em En
| MEDLINE
| ID: mdl-32444666
ABSTRACT
A novel Bacillus licheniformis strain (DM-1) was isolated from a mature reservoir in Dagang oilfield of China. DM-1 showed unique properties to utilize petroleum hydrocarbons and agroindustrial by-product (molasses) for exopolysaccharide (EPS) production under oil recovery conditions. The DM-1 EPS was proven to be a proteoglycan with a molecular weight of 568 kDa. The EPS showed shear thinning properties and had high viscosities at dilute concentrations (<1%, w/v), high salinities, and elevated temperatures. Strain DM-1 could degrade long-chain n-alkanes up to C36. Viscosity reduction test have shown that the viscosity of the crude oil was reduced by 40% compared with that before DM-1 treatment. Sand pack flooding test results under simulated reservoir conditions have shown that the enhanced oil recovery efficiency was 19.2% after 7 days of in-situ bioaugmentation with B. licheniformis DM-1. The obtained results indicate that strain DM-1 is a promising candidate for in situ microbial enhanced oil recovery (MEOR).
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
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Biodegradação Ambiental
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Petróleo
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Alcanos
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Campos de Petróleo e Gás
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Bacillus licheniformis
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Hidrocarbonetos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article